fth1 d1d4 rabbit mab  (Proteintech)

Journal: International Journal of Molecular Sciences

Article Title: Distinct TP53 Mutation Types Exhibit Increased Sensitivity to Ferroptosis Independently of Changes in Iron Regulatory Protein Activity

doi: 10.3390/ijms21186751

Figure Lengend Snippet: Erastin treatment differentially affects TFRC and ferritin heavy chain 1 (FTH1) protein expression in cells expressing distinct TP53 mutation types. Representative images and quantification of Western blots examining the expression of ( A , B ) transferrin receptor 1 (TFRC) and ( C , D ) ferritin heavy chain 1 (FTH1) in TP53 null H1299 cells (H1299) or H1299 cells transfected with wild-type (WT) TP53, or the indicated TP53 mutant following treatment with DMSO (control) or 10 µM erastin for 24 h ( n = 3–4 per cell type and treatment). Relative expression was normalized using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a loading control. * Denotes statistical differences from respective control, p < 0.05. Error bars indicate SEM.

Article Snippet: The membranes were incubated with primary antibodies diluted in the 5% NFDM-T at the following concentrations: CD71 (sc-9099) (H-300) (Santa Cruz Biotechnologies, Santa Cruz, CA, USA) at 1:100 dilution, CD71 (D7S5Z) #13208 (Cell Signaling Technology, Danvers, MA, USA) at 1:1000 dilution, FTH1 (D1D4) #4393S (Cell Signaling Technology, MA, USA) at 1:1000 dilution and loading control glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (sc-47724) (0411) (Santa Cruz Biotechnologies, Santa Cruz, CA, USA) at 1:1000 dilution.

Techniques: Expressing, Mutagenesis, Western Blot, Transfection, Control

Journal: Cell Reports

Article Title: Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning

doi: 10.1016/j.celrep.2020.108076

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-FTH1 (D1D4) , Cell Signaling , Cat#4393S; RRID: AB_11217441.

Techniques: Software, Virus, Recombinant